DNA-Based Services to Enhance Operational Efficiency
DNA is nature’s storage mechanism, encoding vast amounts of data about the health, history, and potential of entire ecosystems. And that data is underutilized.
DNA is everywhere. As the blueprint of life, you can find DNA wherever life exists, be it microbial, plant, or animal. Even in the harshest areas of your mine site, DNA’s pervasiveness is a powerful asset: soil, water, waste rock, dust, even tailings all carry genetic signals that reveal microbial activity, ecosystem health, and environmental change.
Using genetic sequencing and barcoding to study DNA, Koonkie can interpret this invisible layer of data from your site. We use this data to pinpoint the organisms that already thrive in specific mining conditions, and then use their DNA to decode how to influence them to increase yield, purity, and much more!
Koonkie finds native microbes that can increase biomining yields, reduce toxicity in tailings, help prospect for new ore deposits, serve as an indicator of environmental health, or we can customize our search to find organisms to help meet specific operational or regulatory needs.
Stages of a Mine’s Life Cycle
Exploration
Development

Compared to other bioindicators like animals and plants, microbes are early, accessible, and sensitive sensors of environmental changes. Using microbes for environmental assessment of mining practices can replace or bolster existing methods. Establishing strong soil baseline data allows microbes to be used as a monitoring tool that allows companies to intervene early, ultimately improving sustainability efforts, saving costs, and ensuring that regulatory requirements are met.

Compared to other bioindicators like animals and plants, microbes are early, accessible, and sensitive sensors of environmental changes. Using microbes for environmental assessment of mining practices can replace or bolster existing methods. Establishing strong baseline data for local water sources allows microbes to be used as a monitoring tool that allows companies to intervene early, ultimately improving sustainability efforts, saving costs, and ensuring that regulatory requirements are met.

Waste rock and tailings are sources of acid mine drainage, where sulfur compounds oxidize to create highly acidic runoff. Neutralization by sulfate-reducing bacteria can prevent environmental contamination. Koonkie can help sample for and identify key target species that are already adapted to your mine's specific conditions.
Operations

Biomining (or bioleaching) is an established, eco-friendly approach to metal extraction that uses naturally occurring microbes. Instead of relying on energy-intensive smelting or harsh chemicals, biomining harnesses the power of bacteria to break down rock and release metals like copper, gold, nickel, and rare earth elements.

Native microbes with unique metabolic capabilities can be used to remediate harmful mining byproducts, or recover valuable metals that have leached into tailings. The majority of microbes have not yet been discovered. Identifying these specific microbes and harnessing their metabolic potential by implementing targeted growth strategies can serve as an effective and ecologically friendly bioremediation and recovery strategy.

Certain microbes have the ability to solidify the materials found in tailings, producing a natural cement. This process, called biocementation, is an environmentally friendly, cost effective, and more durable solution to stabilize tailings than current methods. Putting microbes with biocementation capabilities to work is an emerging strategy for controlling fugitive dust from tailings. Even better, microbes with these capabilities are often already present in tailings. Biocementation has been shown to strengthen and stabilize soil, sand, and concrete.
Closure & Reclamation

By monitoring the microbial communities in tandem with insects, plants, and animals at reclaimed mining sites and comparing them to undisturbed sites nearby, we can learn about the effectiveness of the reclamation strategies, even at early stages of efforts that span decades. Using microbes as biosensors gives companies timely insights that allows them to adjust current efforts and inform future reclamation strategies. This tool is a streamlined, cost-effective approach that helps ensure that regulatory requirements are met.

Native microbes with unique metabolic capabilities can be used to remediate harmful mining byproducts, or recover valuable metals that have leached into tailings. The majority of microbes have not yet been discovered. Identifying these specific microbes and harnessing their metabolic potential by implementing targeted growth strategies can serve as an effective and ecologically friendly bioremediation and recovery strategy.

Microbes can be harnessed to reduce greenhouse gasses by carbon capture (converting CO2 to stable carbonates) and oxidation of methane, which can be emitted from waste rock. Koonkie can help sample and measure baseline carbon content while simultaneously searching for microbes that increase carbon capture potential, allowing for informed, actionable insights.

From something as simple as a grab sample, DNA barcoding can be quickly and affordably used for quantitative tracking of complex communities that aren’t observable by the naked eye, revealing information about thousands of community members from a single sample. Capturing ecosystem diversity, these methods can be used to evaluate the presence of insects, plants, animals, and microbes. As animals live and roam in an environment, they shed cells and DNA that can be used to identify and track their activity. Environmental DNA (eDNA) testing is transforming biodiversity monitoring, allowing groups like Koonkie to provide a snapshot of nearly all living species in an ecosystem from streamlined soil or water samples.
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